Abidah M. T1., Dutsinma U. A1., Kawo A. H1., Kaosara A. O1., Bashir M2.
1Department of Microbiology, Bayero University, Kano
2Department of Microbiology, Modibbo Adama University, Yola
*Corresponding author’s Email: amtukur@buk.edu.ng, doi.org/10.55639/607.02010048
ABSTRACT
Biofilm are structured communities of microorganisms embedded in a self-produced matrix that enhances survival under extreme and harsh conditions. In Staphylococcus aureus, biofilm formation contributes to persistent infections and increased resistance to antibiotics. This study aimed to assess the biofilm production capacity of S. aureus isolated from clinical and community settings within Kano Metropolis using Tissue culture method and Tube method. Sixty (60) S. aureus isolates were obtained from patients, healthcare workers, apparently healthy individual, food handlers and surgical equipment. Methicillin resistance was determined using EUCAST disk diffusion method (Cefoxitin 30 μg) and Biofilm production was assessed using both TCP and Tube methods. Among the 60 Staphylococcus aureus isolates, 24 (40.0 %) were MRSA and 36 (60.0 %) were MSSA. TCP method detected biofilm production in 43 isolates (71.70 %) while TM identified 31 (51.70 %) biofilm producers. MSSA showed higher frequency of biofilm production compared to MRSA using both TCP and Tube methods. Biofilm production was most prevalent among isolates obtained from surgical equipment (100 %) and less among isolates obtained from healthcare workers (57.1 %). TCP methods was more sensitive than Tube Method in detecting biofilm production, there is a slight agreement (k~0.15) between Tissue culture plate method and Tube method suggesting that the methods classified the isolates differently. MSSA isolates had slightly higher biofilm production tendency and surgical equipment appears to be a significant reservoir for biofilm producing S. aureus underscoring the importance of infection control practices.
Keywords:
S. aureus,
MRSA,
MSSA,
Biofilm,
TCP.