H. Sule and A. I. Abdulkadir
1Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Bayero University, Kano-Nigeria
*Corresponding author’s Email: sule.hamza@yahoo.com, doi.org/10.55639/607.02010064
ABSTRACT
The research aimed to isolate and identify possible fungal and bacterial contaminants associated with these surfaces in Faculty of Allied Health Sciences, Bayero University, Kano. In the study protocol, tap and door handles surfaces were swabbed using sterile swab stick and inoculated onto Potatose Dextrose Argar (PDA), Nutrient gar and chocolate agar and incubated at 37oC for yeast and bacteria and 28 oC for moulds. Positivity rate of the different sample sources showed that tap handles (for toilet had 17.5%, laboratory had 16.7%) while for the door handles (that of toilet had 21.1% while offices had only 7% as the least); also for the door handles, interior had 57.3% while exterior of the door handles had 42.7%. With regards to the isolates, bacteria had 74.7% while fungi had 25.3%. Distribution of the isolate shows: Candida spp had 30.4%, A. fumigatus 21.7%, A. flavus 10.9%, A. niger 6.5%, Penicillium 21.7%, Rhizopus spp 6.5% and Mucors spp had 2.2% isolation rate. We can therefore conclude that tap and door handles harbours a variety of organisms including fungal and bacterial species.
Keywords:
Contamination,
Isolation,
Identification,
Fungi,
Bacteria

